LOCUS       sequence-125369-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY010 (pcDNA3.1-hAsCpf1)
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gccaccatga cacagttcga gggctttacc aacctgtatc aggtgagcaa gacactgcgg
      181 tttgagctga tcccacaggg caagaccctg aagcacatcc aggagcaggg cttcatcgag
      241 gaggacaagg cccgcaatga tcactacaag gagctgaagc ccatcatcga tcggatctac
      301 aagacctatg ccgaccagtg cctgcagctg gtgcagctgg attgggagaa cctgagcgcc
      361 gccatcgact cctatagaaa ggagaaaacc gaggagacaa ggaacgccct gatcgaggag
      421 caggccacat atcgcaatgc catccacgac tacttcatcg gccggacaga caacctgacc
      481 gatgccatca ataagagaca cgccgagatc tacaagggcc tgttcaaggc cgagctgttt
      541 aatggcaagg tgctgaagca gctgggcacc gtgaccacaa ccgagcacga gaacgccctg
      601 ctgcggagct tcgacaagtt tacaacctac ttctccggct tttatgagaa caggaagaac
      661 gtgttcagcg ccgaggatat cagcacagcc atcccacacc gcatcgtgca ggacaacttc
      721 cccaagttta aggagaattg tcacatcttc acacgcctga tcaccgccgt gcccagcctg
      781 cgggagcact ttgagaacgt gaagaaggcc atcggcatct tcgtgagcac ctccatcgag
      841 gaggtgtttt ccttcccttt ttataaccag ctgctgacac agacccagat cgacctgtat
      901 aaccagctgc tgggaggaat ctctcgggag gcaggcaccg agaagatcaa gggcctgaac
      961 gaggtgctga atctggccat ccagaagaat gatgagacag cccacatcat cgcctccctg
     1021 ccacacagat tcatcccc
//
