LOCUS       sequence-125374-        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized LbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY016 (pcDNA3.1-hLbCpf1)
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(58..138)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(145..192)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 aacgggccct ctagactcga gcggccgcca ctgtgctgga tatctgcaga attcttaggc
       61 atagtcgggg acatcatatg ggtatgcata atcaggcacg tcgtagggat aagcgtaatc
      121 tggaacatcg tatgggtagg atcccttttt cttttttgcc tggccggcct ttttcgtggc
      181 cgccggcctt ttgtgcttca cgctggtctg ggcgtactcc agccactcct tgttagagat
      241 ggcgatcttc accttatcca gcttctcgtc ctcggccttc ttgaactggc cgatggccca
      301 cagcaccttt ctggcgatgt tataggcgcc attggcgtcg gcgttctttg gcaggatggc
      361 attctcctgg gcctcatagt tccggctatc gtagaagatg ccgtcggagt tcttcacagg
      421 gctgatcaga aaatccacgt cggtgcggcc tgtgatgctg ttccgcatct gcagcatcag
      481 gctcatcagg gccataaagc tagagtagaa ggccttgtcg gactgctcgc acagcagggc
      541 tctgatatcg ccctgctgat aattgatgcc gtacttgttg aacagctcct tataggcgct
      601 ggtcaggcac acctcctccc agtcgaacac gttgttcttc ttaggattcc ggaagattct
      661 gatccggttg ccgtaggagt acagcttcca cttcttgatg taatcggcgt ctgtgcgaga
      721 gaagttctta tagtccaggg caaactcgaa cagatcctcc tcgggcacgt acatgatcct
      781 gtcaaaggag ctgatgaact tcttggaatc ggcgatgctg gtatacttgg ttttcagcag
      841 gttcacaaag ccggtagatg gatcgatctt ggatgtcagc caggcaggga tgtaaaagat
      901 gaagccgttc tgggtagaca tggacttaaa gctctcgaac ttattggtga tctgatagcc
      961 cttcagggcg ccgcctgttg cacaaggatt agacttcttg tccaccatgt agttcagctt
     1021 atcgatcagc atcttctcga acttctgata cacctgcttc tccaccttca cgcggctatt
     1081 cttaaagcca gagttcaggt cctccagggc gatcacggca tcgta
//
