LOCUS       sequence-125376-         497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PHA metabolic engineering, similar to pJRT9, with R388 oriT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 497)
  TITLE     IMCE attB donor plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJRT16
FEATURES             Location/Qualifiers
     source          1..497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..72)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctttgctag ccattattaa tctcctcaat caatcagatc gtttaaactc atcagtggtg
       61 gtggtggtgg tgaagtgcca atgcatggtg gtcctggacg tagtggtaac gcggtcggct
      121 atcgtgctgg gccggtttca ggaaccccat cagtgcgttg cggctgtctc ggcaggcggc
      181 tttgtgttcc atgtcgagaa agtggccggt ggcctgaagg gtcgtgaagg tggcgtgctg
      241 cacgtggttg gcgaacaacc gtgcgccatc ggcggcggtg tattcgtccc attcgccgtt
      301 catgaacagc accggcacgt tgattttttc cgccgctttg aaaaagcatt ggcgatcgct
      361 gtgcagcagg tcgctgatgt ggaagtgcat ctgcccgtat tcatgatcgg ccaggctgct
      421 gacgtgccga tagttgaagc gcttgaacag ggtcggcagg tgtttgccga tggtgctgtt
      481 caccaggttg ccgaccc
//
