LOCUS       sequence-125378-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V2m with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-279
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(61..78)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(97..246)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(359..370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tggccacacc agccaccacc ttctgatagg cagcctgcac ctgaggagtg cggccgctta
       61 atggtgatgg tgatggtggc cagatccaca attaattaac atatcgagat cgaaatcgtc
      121 cagagcatcg gagccgagca tgtccagatc aaagtcatcc aatgcgtcgc ttcccaacat
      181 atccaggtca aagtcatcga gggcatcaga acccagcatg tcgagatcga aatcgtccag
      241 cgcgtcggcg cgccctccgc tgcccttctc ggtcatggtg caagcatgga cgacaacctg
      301 gttggcaagc aaaaactgat gatcagaatc atcagataaa gtaatcccat aataatcgtc
      361 ttccttcaat tcttgtaact cgaaataaaa tccgcggcac tcacgtgcaa aagcagcggc
      421 gggagcaggc ctgaattttt tagagccggc acacttcgaa agaacgttaa gcaaaactga
      481 tccgcctcct gatccgccac ctgatccgcc cccagatccc ctcaggccat aaagaattgg
      541 agcgtaagtc tggtaggtag ctttacgaac atgggaaccc aacagagaaa gatctctggc
      601 ctcaatagtc cactcaaaat aagctttatt tgaagccttt ctataggatt ctactaattc
      661 gttggctctc tcaggcccct cagatattgg gtagctcttt gaaacttcct tgacaagctc
      721 aacaattcta ccgtcggggg ctttctttt
//
