LOCUS       sequence-125383-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V1m with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-277
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..85)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(104..253)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(366..377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gggcattggc cacaccagcc caccaccttc tgataggcag cctgcacctg aggagtgcgg
       61 ccgcttaatg gtgatggtga tggtggccag atccacaatt aattaacata tcgagatcga
      121 aatcgtccag agcatcggag ccgagcatgt ccagatcaaa gtcatccaat gcgtcgcttc
      181 ccaacatatc caggtcaaag tcatcgaggg catcagaacc cagcatgtcg agatcgaaat
      241 cgtccagcgc gtcggcgcgc cctccgctgc cgttgcctct ctcgccgcaa gcatggacga
      301 caacctggtt ggcaagcaaa aactgatgat cagaatcatc agataaagta atcccataat
      361 aatcgtcttc cttcaattct tgtaactcga aataaaatcc gcggcactca cgtgcaaaag
      421 cagcggcggg agcaggcctg aattttttag agccggcaca cttcgaaaga acgttaagca
      481 aaactgatcc gcctcctgat ccgccacctg atccgccccc agatcccctc aggccataaa
      541 gaattggagc gtaagtctgg taggtagctt tacgaacatg ggaacccaac agagaaagat
      601 ctctggcctc aatagtccac tcaaaataag ctttatttga agcctttcta taggattcta
      661 ctaattcgtt ggctctctca ggcccctcag atattgggta gctctttgaa acttccttga
      721 caagctcaac aattctaccg tcgggggctt tcttttggcc catctcaaaa gtaataactt
      781 caaaatattc gacaccctta atggtacgag acaaacggcg gacactacga ggtgttctaa
      841 caaccaactc
//
