LOCUS       sequence-125384-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V3 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-280
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..84)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(103..252)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(365..376)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gggcattggc cacaccagcc accaccttct gataggcagc ctgcacctga ggagtgcggc
       61 cgcttaatgg tgatggtgat ggtggccaga tccacaatta attaacatat cgagatcgaa
      121 atcgtccaga gcatcggagc cgagcatgtc cagatcaaag tcatccaatg cgtcgcttcc
      181 caacatatcc aggtcaaagt catcgagggc atcagaaccc agcatgtcga gatcgaaatc
      241 gtccagcgcg tcggcgcgcc ctccgctgcc ccacagcacg ctgtcgcaat tatggacgac
      301 aacctggttg gcaagcaaaa actgatgatc agaatcatca gataaagtaa tcccataata
      361 atcgtcttcc ttcaattctt gtaactcgaa ataaaatccg cggcactcac gtgcaaaagc
      421 agcggcggga gcaggcctga attttttaga gccggcacac ttcgaaagaa cgttaagcaa
      481 aactgatccg cctcctgatc cgccacctga tccgccccca gatcccctca ggccataaag
      541 aattggagcg taagtctggt aggtagcttt acgaacatgg gaacccaaca gagaaagatc
      601 tctggcctca atagtccact caaaataagc tttatttgaa gcctttctat aggattctac
      661 taattcgttg gctctctcag gcccctcaga tattgggtag ctctttgaaa cttccttgac
      721 aagctcaaca attctaccgt cggg
//
