LOCUS       sequence-125386-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the Agrobacterium tumefaciens Nopaline synthase promoter as
            a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPnos  (GB0072)
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        141..320
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     regulatory      391..400
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      395..404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(434..453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(435..453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(460..477)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(460..476)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(469..491)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(704..723)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tatggtcgac ctgcaggcgg ccgcgaattc actagtgatt gggcgatgag tggtctcggg
       61 agatacatga gaattaaggg agtcacggta tgacccccgc cgatgacgcg ggacaagccg
      121 ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactgagcc gcgggtttct
      181 ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct
      241 aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa
      301 ttcccctcgg tatccaatta gagtctcata ttcactctca actcgatcga ggcaatgcga
      361 gaccactcat cgcccaatcg aattcccgcg gccgccatgg cggccgggag catgcgacgt
      421 cgggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
      481 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      541 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      601 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      661 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      721 ccttcctttc tcgccacgtt cgccggcttt cc
//
