LOCUS       sequence-125387-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Dundr M, Ospina JK, Sung MH, John S, Upender M, Ried T, Hager GL, 
            Matera AG
  TITLE     Actin-dependent intranuclear repositioning of an active gene locus 
            in vivo.
  JOURNAL   J Cell Biol. 2007 Dec 17. 179(6):1095-103.
  PUBMED    18070915
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2007 Dec 17. 179(6):1095-103."
COMMENT     Sequence Label: Cherry-LacRep
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             61..768
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(206..224)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     474..493
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(812..831)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
       61 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
      121 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      181 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      241 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      301 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      361 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      421 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      481 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      541 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      601 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      661 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      721 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
      781 tctcgaggga tggtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
      841 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
      901 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
      961 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
     1021 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
     1081 tcgatggtag aa
//
