LOCUS       sequence-125388-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orthogonal GFP message under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS
  TITLE     Protein synthesis by ribosomes with tethered subunits.
  JOURNAL   Nature. 2015 Jul 29. doi: 10.1038/nature14862.
  PUBMED    26222032
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jul 29. doi: 10.1038/nature14862."
COMMENT     Sequence Label: pT7oGFP
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     105..124
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        105..123
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             808..855
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             856..879
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
       61 acacaacgtg gctttccccc ccccccctgc aggtcgacgg atcctaatac gactcactat
      121 agggagacta tatctgttat tttttcacac cacagatcta tgagcaaagg tgaagaactg
      181 tttaccggcg ttgtgccgat tctggtggaa ctggatggcg atgtgaacgg tcacaaattc
      241 agcgtgcgtg gtgaaggtga aggcgatgcc acgattggca aactgacgct gaaatttatc
      301 tgcaccaccg gcaaactgcc ggtgccgtgg ccgacgctgg tgaccaccct gacctatggc
      361 gttcagtgtt ttagtcgcta tccggatcac atgaaacgtc acgatttctt taaatctgca
      421 atgccggaag gctatgtgca ggaacgtacg attagcttta aagatgatgg caaatataaa
      481 acgcgcgccg ttgtgaaatt tgaaggcgat accctggtga accgcattga actgaaaggc
      541 acggatttta aagaagatgg caatatcctg ggccataaac tggaatacaa ctttaatagc
      601 cataatgttt atattacggc ggataaacag aaaaatggca tcaaagcgaa ttttaccgtt
      661 cgccataacg ttgaagatgg cagtgtgcag ctggcagatc attatcagca gaataccccg
      721 attggtgatg gtccggtgct gctgccggat aatcattatc tgagcacgca gaccgttctg
      781 tctaaagatc cgaacgaaaa aggcacgcgg gaccacatgg ttctgcacga atatgtgaat
      841 gcggcaggta ttacgtggag ccatccgcag ttcgaaaaat aagtcgaccg gctgctaaca
      901 aagcccgcgg ccgctgaaga tcgatctcga c
//
