LOCUS       sequence-125389-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Orthogonal GFP message under T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Orelle C, Carlson ED, Szal T, Florin T, Jewett MC, Mankin AS
  TITLE     Protein synthesis by ribosomes with tethered subunits.
  JOURNAL   Nature. 2015 Jul 29. doi: 10.1038/nature14862.
  PUBMED    26222032
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jul 29. doi: 10.1038/nature14862."
COMMENT     Sequence Label: pT7oGFP
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..66)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(67..114)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 atcttcagcg gccgcgggct ttgttagcag ccggtcgact tatttttcga actgcggatg
       61 gctccacgta atacctgccg cattcacata ttcgtgcaga accatgtggt cccgcgtgcc
      121 tttttcgttc ggatctttag acagaacggt ctgcgtgctc agataatgat tatccggcag
      181 cagcaccgga ccatcaccaa tcggggtatt ctgctgataa tgatctgcca gctgcacact
      241 gccatcttca acgttatggc gaacggtaaa attcgctttg atgccatttt tctgtttatc
      301 cgccgtaata taaacattat ggctattaaa gttgtattcc agtttatggc ccaggatatt
      361 gccatcttct ttaaaatccg tgcctttcag ttcaatgcgg ttcaccaggg tatcgccttc
      421 aaatttcaca acggcgcgcg ttttatattt gccatcatct ttaaagctaa tcgtacgttc
      481 ctgcacatag ccttccggca ttgcagattt aaagaaatcg tgacgtttca tgtgatccgg
      541 atagcgacta aaacactgaa cgccataggt cagggtggtc accagcgtcg gccacggcac
      601 cggcagtttg ccggtggtgc agataaattt cagcgtcagt ttgccaatcg tggcatcgcc
      661 ttcaccttca ccacgcacgc tgaatttgtg accgttcaca tcgccatcca gttccaccag
      721 aatcggcaca acgccggtaa acagttcttc acctttgctc atagatctgt ggtgtg
//
