LOCUS       sequence-125390-        3352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the Agrobacterium tumefaciens Nopaline synthase promoter as
            a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPnos  (GB0072)
FEATURES             Location/Qualifiers
     source          1..3352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(42..221)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(402..420)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(403..420)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(438..454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(438..454)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(451..473)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    462..478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(486..492,493..510,511..516))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    531..552
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(669..686)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(840..1428)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(920..939)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1599..2390,2391..2459))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2222..2241
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2460..2564)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2642..3097)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2779..2800)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2991..3010
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3223..3245
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3237..3254
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3238..3254
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3261..3280
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3261..3279
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3310..3319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3314..3323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcattgc ctcgatcgag ttgagagtga atatgagact ctaattggat accgagggga
       61 atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct
      121 taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc
      181 cagaaacccg cggctcagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa
      241 acggcttgtc ccgcgtcatc ggcgggggtc ataccgtgac tcccttaatt ctcatgtatc
      301 tcccgagacc actcatcgcc caatcactag tgaattcgcg gccgcctgca ggtcgaccat
      361 atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat
      421 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      481 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      541 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      601 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      661 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      721 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      781 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      841 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      901 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      961 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1021 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1081 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1141 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1201 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1261 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1321 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1381 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1441 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1501 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1561 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1621 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1681 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgc
     1741 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1801 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1861 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1921 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1981 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2041 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2101 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2161 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2221 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2281 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2341 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2401 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2461 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2521 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2581 gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2641 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2701 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2761 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2821 aacgtcaaag ggcgaaaaac cgtctatcag ggcgctggcc cactacgtga accatcaccc
     2881 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     2941 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3001 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3061 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca
     3121 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     3181 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     3241 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggc ccgacgtcgc
     3301 atgctcccgg ccgccatggc ggccgcggga attcgattgg gcgatgagtg gt
//
