LOCUS       sequence-125391-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sensor pair 1 V1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pVITRO1-SS-267
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..97
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             98..118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             832..843
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tcttttaacc cttttttagg tgttgtgaaa accaccgcta attcaaagca accggtgata
       61 tcaaggatcc atggactaca aagacgatga cgacaagcct aagaagaaac ggaaggtgtc
      121 cagacctggg gagaggccat tccagtgtcg gatctgtatg agaaatttta gcactatggc
      181 tgtactgagg agacatacaa gaacccatac aggcgagaag ccctttcagt gcaggatctg
      241 catgcgaaac ttttctcgca gagaagtgct tgaaaaccac ctgcggactc ataccggcga
      301 aaaaccattt cagtgtcgaa tttgcatgcg caactttagc cagacagtga acttggatag
      361 acaccttaag actcatctga ggcagaagga cggggagaga cctttccagt gcagaatctg
      421 catgagaaac ttttctaaga aagatcatct gcaccgccat acccgcaccc acaccggcga
      481 aaaacccttc cagtgccgca tctgtatgcg aaattttagc cagaggccac atcttacaaa
      541 tcaccttaga acacacactg gagaaaaacc atttcagtgt cggatctgca tgcgcaactt
      601 ctccgtgggc gccagcctca aacgccacct gaaaacccac ctgaggggat ctgggggcgg
      661 atcaggtgga ggctcaggag gtgggtccgg aggtggatct ggtggcgggt ccggaggcgg
      721 atccgttttg cttaacgttc tttcgaagtg tgccggctct aaaaaattca ggcctgctcc
      781 cgccgctgct tttgcacgtg agtgccgcgg attttatttc gagttacaag aattgaagga
      841 agacgattat tatgggatta ctttatctga tgattctgat catcagttt
//
