LOCUS       sequence-125394-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CIS-V2 with N-FLAG and C-HIS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Slomovic S, Collins JJ
  TITLE     DNA sense-and-respond protein modules for mammalian cells.
  JOURNAL   Nat Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585.
  PUBMED    26389572
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Sep 21. doi: 10.1038/nmeth.3585."
COMMENT     Sequence Label: pBW121-SS-278
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..86)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..254)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(367..378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagggcattg gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgcg
       61 gccgcttaat ggtgatggtg atggtggcca gatccacaat taattaacat atcgagatcg
      121 aaatcgtcca gagcatcgga gccgagcatg tccagatcaa agtcatccaa tgcgtcgctt
      181 cccaacatat ccaggtcaaa gtcatcgagg gcatcagaac ccagcatgtc gagatcgaaa
      241 tcgtccagcg cgtcggcgcg ccctccgctg cccttctcgg tcatggtgca attatggacg
      301 acaacctggt tggcaagcaa aaactgatga tcagaatcat cagataaagt aatcccataa
      361 taatcgtctt ccttcaattc ttgtaactcg aaataaaatc cgcggcactc acgtgcaaaa
      421 gcagcggcgg gagcaggcct gaatttttta gagccggcac acttcgaaag aacgttaagc
      481 aaaactgatc cgcctcctga tccgccacct gatccgcccc cagatcccct caggccataa
      541 agaattggag cgtaagtctg gtaggtagct ttacgaacat gggaacccaa cagagaaaga
      601 tctctggcct caatagtcca ctcaaaataa gctttatttg aagcctttct ataggattct
      661 actaattcgt tggctctctc aggcccctca gatattgggt agctctttga aacttccttg
      721 acaagctcaa caattctacc gtcgggggct ttcttttggc ccatctcaaa agtaataact
      781 tcaaaatatt cgacaccctt aatggtacga gacaaacggc ggacactacg aggtgttcta
      841 acaaccaact catgggtcgc attacacgta aacttgagta attctggcac ttcacgactt
      901 gagtcacttt tg
//
