LOCUS       sequence-125399-        5062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the S. Lycopersicum metallothionein II-like protein (MTB) 
            gene promoter as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPMtb  (GB0080)
FEATURES             Location/Qualifiers
     source          1..5062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             83..94
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(2112..2130)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2113..2130)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2148..2164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2148..2164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2161..2183)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2172..2188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2196..2202,2203..2220,2221..2226))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2241..2262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2379..2396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2550..3138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2630..2649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3309..4100,4101..4169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3932..3951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4170..4274)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4352..4807)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4489..4510)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4701..4720
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4933..4955
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4947..4964
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4948..4964
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4971..4990
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4971..4989
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5020..5029
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5024..5033
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcattga tgattggtct gaaacaagaa ttgagaaatc aaatgtgatt tttttttttt
       61 ttgattggtg tagaaatgag tgattgaggg aagaagcatg agtgtctatt tatagtgaaa
      121 cgaaggtaca tggatgatat taaccgtaca cgtgtcgtga tcgtatggga atatgttatc
      181 cttgtaagtc ggtccataca tgatcctaat ttcatccacg tattcgtcgt tttctataat
      241 cacaattacc attcgtacgt atgttctaga catatttaca ttctctatcg accgcgactt
      301 atttatctat atcgaatgtt tataacaaac taaataactt tatattttga cacttttcaa
      361 gatttaaatg attttatctt tgattgtaaa ttttttatat ttatttttat ttattttgaa
      421 ttaacagtta ttgtgaccta tagtactttt tatatagttg tataaatttt attttaaaaa
      481 atagaaaatt tcgcgcgtat gttttcggtc aaactttaat tatttgactc ttgaaaaacg
      541 aaaagtgtca cataaatcga aacaaatgaa gtatattgtg atcgagctga tgtagcatat
      601 gagatgatag gtgcgtttgg gtggtggtca gggatcggct aaaaaaataa tttagaatta
      661 aattttttta aaaagattgt tgtttttcct aaaataaaaa tgtaatttta aatttgagga
      721 gagtttggaa aaatgttttc cttaattttt taaaggaaat catttttctt aattttgagg
      781 aaaatgagtt gattcgaaaa acttttttca aaatttttgt cccaaccaaa catgagaaaa
      841 ttggaaaaca ctttctggtt ccttcatacc aaacacactc gatatcaaga aagattaggg
      901 ttcaatttaa tctgttatgt cattttaatt gaattcatca ggttcaattt atttaatacg
      961 atatatatta ttaatgtgat ttgtaaattc ttatacaaaa ataagttact taatatgcta
     1021 taaaaaagta acaactacga atttctcgat aaatttctga attaatcgac caaattgata
     1081 gtgacttctt atctacccaa ggaaataaca ttttctgttg gaacaaattg tgggcatgca
     1141 cgtaccttgg tttaagttgt taatgtttat tctttttatt tatctcaaat aatatgatat
     1201 ttatttaaat aatttaacat gtaaaaatta aaattatttt tcaaaaagtg atgataaaat
     1261 ttaaatattt atgttacata ggaaacaaaa ataataattc aatatagtac ttttagcata
     1321 aaaatagtta atgtttacgt ttcgagaata tttttttcac gtattatttt ttctctagaa
     1381 cttggtagat accaataatt tgtggcccaa aatctacaca agtatgttca taattgacta
     1441 cacaagttta tgtttaatat gattaattaa gttgtgttta atgaataaga tgatcattga
     1501 agagtattga taaaaaatat tgtaagataa ttaaataagt tctaatttta atagaaaatg
     1561 aagaattaaa tgattatttt gttaatgaaa tgtgtttaat tatatacgat aaaatgatta
     1621 agaaatgtgt agatgtcatg cttgttcaat cttcctcttt gtataagtag cttgatataa
     1681 taacttcata ttattacaca tccaagataa aagtatttct agatgaattt gtactttgac
     1741 acattcaatt aattaatgac atagttatgt cattattcgt tatgtataca aagtagtatg
     1801 tcagaaataa tgtattttcc gctaaaaata atgatacttt gtagatatga tatgttttga
     1861 ctttgtgact tttttgtttg tatctcaaaa tttagtgact ttgggttaga gaaactagtt
     1921 atataacttt tgtatgaaac gcaaatacta atagttactt gatcgatatg aagtttgttc
     1981 aaatattaat aaaatcaaaa agtaagatac tcccgagacc actcatcgcc caatcactag
     2041 tgaattcgcg gccgcctgca ggtcgaccat atgggagagc tcccaacgcg ttggatgcat
     2101 agcttgagta ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt
     2161 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     2221 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     2281 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     2341 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2401 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2461 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2521 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2581 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2641 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2701 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2761 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2821 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2881 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2941 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3001 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3061 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3121 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3181 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3241 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3301 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3361 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3421 tctggcccca gtgctgcaat gataccgcgc gacccacgct caccggctcc agatttatca
     3481 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3541 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3601 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3661 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3721 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3781 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3841 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3901 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3961 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4021 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4081 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4141 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4201 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4261 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca
     4321 cagatgcgta aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa
     4381 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     4441 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     4501 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     4561 ggcgctggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     4621 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     4681 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4741 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4801 ggcgcgtcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     4861 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     4921 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact
     4981 cactataggg cgaattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga
     5041 attcgattgg gcgatgagtg gt
//
