LOCUS       sequence-125403-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfer plasmid for Tet-regulated shRNA expression with 
            a TREtight promoter. Contains DsRed2 and Venus fluorescent 
            reporters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe 
            SW
  TITLE     Toolkit for evaluating genes required for proliferation and survival
            using tetracycline-regulated RNAi.
  JOURNAL   Nat Biotechnol. 2011 Jan . 29(1):79-83.
  PUBMED    21131983
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2011 Jan . 29(1):79-83."
COMMENT     Sequence Label: TtRMPVIR
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(10..509)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(58..77)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     451..474
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     ncRNA           complement(532..658)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(686..700)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(728..849)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gctgcaggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt
       61 ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag
      121 cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa
      181 ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt
      241 gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga
      301 cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa
      361 ggggccacca aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg
      421 ccagaggcca cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga
      481 ctgccttggg aaaagcgcct cccctacccg gtagaattgc tagaattgaa aaaagtgatt
      541 taatttatac cattttaatt cagctttgta aaaatgtatc aaagagatag caaggtattc
      601 agttttagta aacaagataa ttgctcctaa agtagcccct tgaattccga ggcagtaggc
      661 ataggaatta taatgcttat ctatacatct gtggcttcac tatagataag cattataatt
      721 cctgcgctca ctgtcaacag caatatacct tctcgagcct tctgttgggt taacctgaag
      781 aagtaatccc agcaagtgtt tccaagatgt gcaggcaacg attctgtaaa gtactgaagc
      841 ctcattcaaa caattaccct gttatcccta gtcgaggctt agctggccgc tacaggaaca
      901 ggtggtggcg gccctcggtg cgctcgtact gctccacgat ggtgtagtcc tcgttgtggg
//
