LOCUS       sequence-125420-        7177 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a
            level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pPUbc  (GB0145)
FEATURES             Location/Qualifiers
     source          1..7177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(4227..4245)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4228..4245)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4263..4279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4263..4279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4276..4298)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4287..4303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4311..4317,4318..4335,4336..4341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4356..4377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4494..4511)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4665..5253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4745..4764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5424..6215,6216..6284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6047..6066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6285..6389)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6467..6922)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6604..6625)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6816..6835
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7048..7070
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7062..7079
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7063..7079
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7086..7105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7086..7104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7135..7144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7139..7148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgggagtt attcttatta tttttgtatg agaaaatgat atcacataca cacttatata
       61 aatttgagta atatcatgaa tcttttttta aaaaaattaa cttctatatt ctcttaaact
      121 aattttcaat cataaatctt tttttaaaaa aatttaactt ctatattctc ttaaactaat
      181 tttcaatcat aaattaaaat aacatttata aactttttaa ttgactacaa aaatctcctc
      241 aatgtccacc agatgactcc acctttttct ccataacttg gaaatagggt cctacaagat
      301 tcccatttcc accaaagaga atagggactc tgtttttatt ttttcttcct tgtggttgga
      361 gtactaaaga ttccattttt gtcccttcta atggaaaata aataatacta ctgagtaagt
      421 aaaaaaaaaa ataatatcgg agataggtga ttaactaaga tataatataa ctttatagct
      481 taataagaaa ttatgagttt acaaaatcca caactcaatt tataaactcg agctaagaaa
      541 aaagagggaa aaaatgaatt taatcattat tttttgtgtt atatttataa tgagcattag
      601 acgcacttaa ttttattgtt tcaatttata aaatatcttt gaattgagtc ttagcaaact
      661 atgtaagctc atcatcaatt aaacactagc gagtcaacta actaatgaat tagtaactaa
      721 ctaatagaat gaacaataac taatccatta gatttattgt gtgatacaat caactattgg
      781 atcgtgttta ttatcaaatg tatgatttta aaattttttt tagaggaaaa attactgaaa
      841 tatatctctt atattttttt tatttttcta aaaaccttta aatttttttt ttaataatgt
      901 attcgagtta catattatgt attcagttta ttttataatg tatccgagtt atatattatg
      961 tattcaattt atgttatgat atatccgtaa tactctttaa tgtattcgag ctacatatta
     1021 atgcattcgg tttgtgttac tttttaagga attaatgtaa ttataattta aagagagata
     1081 gattgtaatt tcatattaaa actatgtgat ttctgtaaaa aatttaaaaa tatatatagg
     1141 gagataacga ttagaacata attaattagg taattgacaa tcatctaact taattatagg
     1201 tactagcgat cttagttttc aaaagttaaa tttacaaatt ttagattaaa catcattttt
     1261 agatatcgag gactagttca ttgtcttcca taataacaaa cacaataatt ggtatttttt
     1321 ttatttatgg gtgatattgt tatgtaggat aaatatgttt atttatttaa tattatataa
     1381 aatatttgtt tcatttattt ttatttatta cgtgtcgctt tttaaaaatt aacttaacta
     1441 atttcaaagt taaattagat atcagtaatt tgataatttc atcaaaaaat ttagatattc
     1501 aaaaactata agaaaagtat tataaattac aatttttttc atatcaatat gataaaaaaa
     1561 tacgatttaa aatactaacc aaaattttta taatttaact ctaaaaataa aaattataac
     1621 aataaaatat gaataaaaat aatattattt atacacaatt aaaattaaaa atcataatta
     1681 ccgaccgaaa cttatgggat aggaaaaaac aatagagcaa cgtataaaaa ggtggagtta
     1741 gcgttgatct tacgccaaaa ttatgacttg tacaagagga ttctagaagc tggaccctac
     1801 tgaatacttc ttttataaca ctccaatttc tcattttaat ctcctcttct tccatttctt
     1861 tcaaaattaa agtattgtta ctctgctatt ggctcaaaac ctctgcaatc tccgactcct
     1921 tcaatttcaa ctcaagcaaa tccacctctt tcactagttt catcactttc aggtacttca
     1981 aaattcacag atctagtgtt tttttgaatt tttttgagag agttttggat agctacttcc
     2041 atgattttga gtgagactaa attttttacc aatttgttaa atattagttt tatatataca
     2101 gtactgtatg atgagctgaa ggaagaattt gtatatggat atcaacttga atgtatggtt
     2161 tatttgagga acgaagcaga tctagttttt ttttttgtta ttagcaaaat taattaattt
     2221 ttggataaat tttagctgct aattcgggaa tcagagacgg tggattagaa gctagttaag
     2281 ttaattgtat gtggagtttt ttttatacta ctaattttaa catatacaaa attgtgtgat
     2341 gagttgaagg aagtatttgt atatgtttat ccacttgaat taggcggatc tagcttattt
     2401 gaaatttttt taataggttt agatagctaa ttccaggatt ttgagtgagt tgaatctgag
     2461 ctactcaatt ttactagatg tgcagttttt agttgtagtt ttacatgtac agtattgtgt
     2521 cacgagctga aggcagaatt tgtttatgga tgtccacttg aatgtatggt tttattcatg
     2581 gaacgaagta gatctagagt ttttggttat tagcaattaa ttagttttag tttagctgca
     2641 aattcgggat taagagctag taaagttaat tgtatgtgaa ttttaatttt tacatataca
     2701 aaattgtttg atgagctgaa cgaagtatct atatatatct atccacttga attaggcaga
     2761 tctagcttct tttttttttt gaacagtttt ggatatctaa ctgtaggatt tagagtgagt
     2821 tgattctgag ctactaagtt ttactatatg tgcaattttt taaacattag ttttatatgt
     2881 acaattttgt gtcatgagct aaaggcaaaa atttgtatat ggatttccac tagcatgtat
     2941 gattttgtta agcagatata tgttgttttg tgttattaac aattgatcag tttttggata
     3001 cgtcatagtg gctaattcgg gatgtagagt cagtggatta agagctagta aagttaattg
     3061 taggtgcaac tttttaatat tgatttttac atatacaaaa ttgtgttatg agctgaagga
     3121 agtatttgtt tatatgtacc cgctcgagtt aggcagatct tgctttttta ttttctttgt
     3181 tacaattatt taacagattt tgatagctaa ttccgtgatt tagagtgagt tgattctgag
     3241 ctactaaatt ttactatatg tgcaattttc taaaattcat ttaagtgtac aattttgttt
     3301 cacgacctga aggcagtatt tgtatatgga tatccactcg aatatatgat tttatttgac
     3361 gaatgaagga gatctagttt ttttatatta acaattaatt gtttttgggt acgtcttagc
     3421 tgctaatttg ggattgagag taagtggatt cagagctagt aaagttaact gtatttgcaa
     3481 atttttaata ttatttttgc ataaacagaa ttgtgtgacg agctgaaagc agtagttgta
     3541 tatactttcc tactggagtg tgtgatttta ttaaggaatg aagcagatct ttttttttag
     3601 ttattagcaa ttaattagtt tttggattac gccttagctg caaattcggg atttagagtc
     3661 agtggattct gggctagata agctaatagt atgtaatatt tactattatt ttgtacatgt
     3721 acaaaattgt gtgatgagct gaaggaagta tagctgtcca ctcgtatgtg tggttctgtt
     3781 cagaaaatta aacagatcta tgtttttctt ttgtgaacaa tcaattagat ttacttgtaa
     3841 ggtttggagt tggtgaattt tgagtgaggt ttttattata gtaaaaataa ttgtatgtaa
     3901 tgtgtttgtc tttatgaatt tgtgatgagc tgaaagtagc aggtgtgata ggtgtgatat
     3961 taatatcttt agaattgcac atgatgtatg tgtttttatt tttatgttgt tgtgttttgc
     4021 atcttttctt tttgttgctt ttgtggaatt tgaaatttgt ggtaaaaggt gtccatttga
     4081 atatcagatc agggtttgga gttgaaggta cggggggcta attgaatgcg agaccactca
     4141 tcgcccaatc actagtgaat tcgcggccgc ctgcaggtcg accatatggg agagctccca
     4201 acgcgttgga tgcatagctt gagtattcta tagtgtcacc taaatagctt ggcgtaatca
     4261 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4321 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     4381 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4441 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4501 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4561 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4621 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4681 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4741 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4801 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4861 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4921 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4981 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5041 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5101 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5161 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5221 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5281 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5341 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5401 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5461 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5521 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5581 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5641 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5701 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5761 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5821 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5881 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5941 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6001 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6061 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6361 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg
     6421 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt
     6481 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     6541 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     6601 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     6661 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     6721 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     6781 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     6841 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     6901 aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc
     6961 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     7021 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     7081 aattgtaata cgactcacta tagggcgaat tgggcccgac gtcgcatgct cccggccgcc
     7141 atggcggccg cgggaattcg attgggcgat gagtggt
//
