LOCUS       sequence-125424-        3895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana heat shock protein 18.2 promoter 
            as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pHsp18.2  (GB0153)
FEATURES             Location/Qualifiers
     source          1..3895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(945..963)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(946..963)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(981..997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(981..997)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(994..1016)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1005..1021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1029..1035,1036..1053,1054..1059))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1212..1229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1383..1971)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1463..1482)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2142..2933,2934..3002))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2765..2784
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3003..3107)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3185..3640)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3322..3343)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3534..3553
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3766..3788
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3780..3797
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3781..3797
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3804..3823
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3804..3822
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3853..3862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3857..3866
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcattga cttttggttc ttgattttcc tgctgatttg atctgattag caaaggacat
       61 atttataggg aagttagagg atgaagagag aatgttcttc gagtttcttg aatagaatag
      121 aaaatgctcc tttttctaaa accttcgctt ggagtcttta gaacacatac acaatgatca
      181 ctgtggtgaa atgaccagat ttttcttggc atttcaggaa gtttcgtttg ttgaaacaag
      241 accgaaatgc aatccttggt tacaattaaa actacgtgcc gttttgcttt gttgttgtat
      301 tatcctcttc gagatacggg ctcagtgaag cggatcttct ttgcaaaagc ccatttctta
      361 ataggtcgga agtggaattt aagaaaatct aactcagaga aaagaggaca aaatcaagat
      421 ttaagattat tagcttttcg ccaaaggaaa actatagtaa gttctcgcaa taacagttgg
      481 cactaaattt ccgagaatgt caacaatctt ttaatggatt tttttgtctc catatataca
      541 agaactgagc cacgtttttg tgacaaacat agacaacata tttactaaac aagtaaacta
      601 aaaaaaaaaa aaatctaaat gattcgattc aagtatcagt tactgtgatc aaaatctcaa
      661 cttatttatt gcctgttcat gtcatgcttg aaccagaaga aatgaccatg gaaagtaatt
      721 gtgattacct ttttgggaaa cttggggtta aaaattgaaa atgtgatatg tcatggtttt
      781 ggtttctaat caatagaact tgcatgaaag aaagaaagaa agagaaatga agagaaaagc
      841 ttctcccgag accactcatc gcccaatcac tagtgaattc gcggccgcct gcaggtcgac
      901 catatgggag agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta
      961 aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1021 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1081 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1141 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1321 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1381 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1501 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1561 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1621 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1681 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1741 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1801 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1861 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1921 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1981 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2041 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2101 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2161 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2221 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2281 cgcgacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2341 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2401 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2461 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2521 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2581 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2641 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2701 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2761 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2821 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2881 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2941 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3001 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3061 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3121 aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     3181 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3241 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3301 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3361 tccaacgtca aagggcgaaa aaccgtctat cagggcgctg gcccactacg tgaaccatca
     3421 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3481 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3541 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3601 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
     3661 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     3721 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     3781 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
     3841 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tgggcgatga gtggt
//
