LOCUS       sequence-125428-        5038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the Arabidopsis thaliana heat shock protein 70B promoter as
            a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pHsp70B  (GB0155)
FEATURES             Location/Qualifiers
     source          1..5038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2088..2106)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2089..2106)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2124..2140)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2124..2140)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2137..2159)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2148..2164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2172..2178,2179..2196,2197..2202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2217..2238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2355..2372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2526..3114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2606..2625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3285..4076,4077..4145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3908..3927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4146..4250)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4328..4783)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4465..4486)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4677..4696
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4909..4931
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4923..4940
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4924..4940
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4947..4966
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4947..4965
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4996..5005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5000..5009
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcatttg ctaaaaaaaa gcttcagtaa ttgaatgaaa ttggaagtga ggtttttcga
       61 tttcttttgt ttgtttatct gtttgatgaa gaatcggaaa cgatggaaga agctatatat
      121 acgaatccga cgagaggaac gagacatttc gagaaaaaag gtcgtggcac aacccagggg
      181 attgtggaaa ggtggagaaa tcgcgcaaac gtacaagaga gttctggatt caccgaccgg
      241 gatatatttt atctccggcg accgcttgat aattaaatgg gcctattaaa gcccatatgg
      301 tataaacttg taggcccgta tatagaataa aaggcccagt gtaagttctg gaggattttc
      361 aatattttgt ttacgtcttc tttcttcact tgttctgtga tttcgaagac gagaaaggaa
      421 aagccatgaa agaggcgaag gagaagaaga atccagaaat ctccgttagt atcacgatat
      481 caacttgggg ggcttttgct gtttatgtga ttactgggtt atttctgatc ttcggatttc
      541 acgtggtccg taacaaatac tctgtcgatc tcatttctga tccaactctc actctgcgtc
      601 tgctatgggt actaaaactt tctttcataa cctggttgct agtggatatt tttcagttaa
      661 ttttagcctt tttgttttga tgttgatatt cagattattg agttcccgat cgtggtaatc
      721 atctacagct tgttccgacg aaacccagag aaatgctcgg tgagtatagt tcatctctga
      781 ggttctctgt gagaattaaa gaaactgaat ttctcgtttt aactcgattg ctttgtgctt
      841 gtctcatttc ccttttatga aattgacaaa acaagctagt atcctcactt tacactctta
      901 atgctcaaaa aaatcgtttc ttttcatata gtattatttt ggtcttgtgt gttttgactc
      961 tgtattcatg attttctcat tgatttccag tactttagag ctgttggccg aagcttagtc
     1021 ggactaattg ccggttagta tttctgtttt tcagcttatt atagctcact aatttttcaa
     1081 agaagttgat tgctcaatga gttcacctgg aaattctaga tgttaagcta aacctgaaga
     1141 tgtgagtgag tctgagcttc ttatgctttc atcttgtgct tttgctgatt cagtatccct
     1201 tgattgtgtc tactatatct atacctgtgt tttgatttgt ttaataggtg ctcttataaa
     1261 tgctttggga gctgtttctt tgggtgcacc tattggaatg cagtatgttt cactaagatc
     1321 ttctttctct atatcttttt atttctttct aattgtggaa atgttgattg taaatctgga
     1381 cttttcagtt gtccagatta aaaatgaggc tccttaatgc tggttgtggt ctgacttctg
     1441 catggagatt gtgtgatttt caaaagctga catgagtatt accttctcat cttatgcaga
     1501 tctctatcaa aaacaattca ctggtccttt ctaatgtctg ttttcacggt aagctgcagt
     1561 gtccatgact taaaagatgt taataccatg ccatatgtgt atttctgcag tttgaaacaa
     1621 accttttgat ggtgtctgat gattttgatt ttcataggtt gtaccagcaa ctgcagtttt
     1681 aggtgcatca tggatagatt ggcatcgtat ctttgcttca ctaaagtaag tcacttatgc
     1741 tacctagtat gataattcac acactttatg ttgttgttaa tccatctttt ttttttcttt
     1801 tagaccaatc ggaattatag aacatatgct cttagttcca gcatatggag caattattgg
     1861 aggatggttt ggagcttggc ctatgccact tgactgggaa agaccatggc aggtaatgtt
     1921 tatttcttct caacatacag tcaagggctt gtttctgaac tttattgctc ccttttttcg
     1981 cagttctccc gagaccactc atcgcccaat cactagtgaa ttcgcggccg cctgcaggtc
     2041 gaccatatgg gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac
     2101 ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2161 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2221 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2341 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2401 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2461 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2521 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2581 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2641 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2701 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2761 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2821 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2881 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2941 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3001 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3061 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3121 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3181 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3241 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3301 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3361 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3421 ccgcgcgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3481 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3541 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3601 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3661 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3721 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3781 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3841 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3901 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3961 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4021 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4081 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4141 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4201 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4261 cgaaaagtgc cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     4321 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4381 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4441 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4501 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg ctggcccact acgtgaacca
     4561 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4621 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4681 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4741 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     4801 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     4861 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     4921 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggcccga
     4981 cgtcgcatgc tcccggccgc catggcggcc gcgggaattc gattgggcga tgagtggt
//
