LOCUS       sequence-125432-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E.coli ORF209/ADPRase in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Gabelli SB, Bianchet MA, Bessman MJ, Amzel LM
  TITLE     The structure of ADP-ribose pyrophosphatase reveals the structural 
            basis for the versatility of the Nudix family.
  JOURNAL   Nat Struct Biol. 2001 May;8(5):467-72.
  PUBMED    11323725
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol."; date: "2001-05"; volume: "8(5)"; pages: "467-72"
COMMENT     Sequence Label: pET11_Ec_ORF209
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             651..673
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    688..712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(712..731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(713..731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagccgga tccttatgcc cactcatttt ttaacgcttg atgatgcagc tgcagccatt
       61 gcaaagcgat gaccgacgct gcgttgtcga ttttcccctc ttctacccac tggtatgcct
      121 gttcccggct taccacatga acgcgaatat cttcgttttc atcagccaga ccgtgaatac
      181 cgcttgcggt cgtggcgtcc acttcgccca ccataattga cgaacgctca ctggtgcccc
      241 ccgggcttgc caggaaactt aacaccggtt tggtccgttt gactatcagt cccgcctctt
      301 caatcgcttc gcgacgggca acatcttcca cactttcacc ctcttcaatc atcccggcaa
      361 ccatctccag tagccaaggg gtttcgctgg tgtcgtacgc ggcaatccga atctgctcaa
      421 tcagcacaac ttcatcacgc actgggtcaa agggtagcaa gactgcggcg tgaccgcgct
      481 caaaaatttc ccgccgcacc tcatgactca tttgcccgtt gaatagacga tgacgaaatc
      541 tataaagatc taatgaaaaa aagccgcgat aaagtgtttc tcgtgcaata atttctacat
      601 cgtttttgcc aaatgtaacg ggcaggttgt ctggcttaag catatgtata tctccttctt
      661 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt
      721 gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtg
//
