LOCUS       sequence-125443-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a neomycin tagged codon optimized L1PA8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Wagstaff BJ, Kroutter EN, Derbes RS, Belancio VP, Roy-Engel AM
  TITLE     Molecular reconstruction of extinct LINE-1 elements and their 
            interaction with nonautonomous elements.
  JOURNAL   Mol Biol Evol. 2013 Jan;30(1):88-99. doi: 10.1093/molbev/mss202. 
            Epub 2012 Aug 23.
  PUBMED    22918960
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/molbev/mss202"; journalName: "Mol Biol Evol"; date: 
            "2013-01"; volume: "30"; issue: "1"; pages: "88-99"
COMMENT     Sequence Label: pBS-L1PA8CHmneo
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     290..309
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
       61 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      121 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc
      181 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
      241 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
      301 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
      361 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
      421 ggggatcggc aataaaaaga caggaataaa acgcacggcc ggccagctag gaattcttag
      481 ctgccgctga tcaggctgaa catccggtgc ttggtcttct gctcctgggt cagcttgctc
      541 aggatgatgg cctccagctt catccaggtg ccggcgaagc tcatgaactc gttccgcttg
      601 atggcggcgt agtactccat ggtgtagatg tgccacatct tcttgatcca gtcgatcatg
      661 ctggggcact tgggctggtt ccaggtcttg gcgatggtga acagggcggc gatgaacatc
      721 cgggtgcagg tgtccttgta gtagaagctc ttgtagtcct tggggtagat gcccagcagg
      781 gggatggcgg ggtcgaaggg gatctcgggc tccaggtcct tcaggaaccg ccacacggtc
      841 ttccacaggg gctgcaccag cttgcactcc caccagcagt gcagcagggt gccgatctcg
      901 ccgcagcccc gccagcaccg gttgttgccg ctcttcttga tgatggccat ccgcacgggg
      961 gtcaggtggt accgcatggt ggtcttgatc tgcatctccc ggatcaccag gctgctgctg
     1021 ctcttcttca tgtgcttgtt ggcggcgtag atgtcctcct tgctgaagtg ccgg
//
