LOCUS       sequence-125497-        1110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parental plasmid for transcription of RNAs with a HdV/M-domain 
            3'-tag for native purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Kieft JS, Batey RT
  TITLE     A general method for rapid and nondenaturing purification of RNAs.
  JOURNAL   RNA. 2004 Jun;10(6):988-95.
  PUBMED    15146082
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-06"; volume: "10(6)"; pages: "988-95"
COMMENT     Sequence Label: pRAV12
FEATURES             Location/Qualifiers
     source          1..1110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      210..257
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(276..292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(276..292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(289..311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    300..316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(324..330,331..348,349..354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    369..390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(507..524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(758..777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cattaagtgg tgttgtgcga aaagaatctc gaagacaagc cggccatggt cccagcctcc
       61 tcgctggcgg ccggtgggca acatgcttcg gcatggtgaa tgggacctct agactgtgca
      121 tcgggtcagg actgaaaggt agcagccctg ggcagttttt tcctgtgcat cgggtcagga
      181 ccttcgggta gcagccctgg gcaggatccc tagcataacc ccttggggcc tctaaacggg
      241 tcttgagggg ttttttgaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      481 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      841 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1081 ttcttgaagt ggtggcctaa ctacggctac
//
