LOCUS       sequence-125512-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untargetted Bacterial Mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Badran AH, Liu DR
  TITLE     Development of potent in vivo mutagenesis plasmids with broad 
            mutational spectra.
  JOURNAL   Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425.
  PUBMED    26443021
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-10-7"; volume: "6:8425. doi"; pages: " 
            10.1038/ncomms9425"
COMMENT     Sequence Label: MP-T4
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca
       61 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
      121 cgtttttttg gacgcgtaca actcaagtct gacataaatg accgctatga gcactgcaat
      181 tacacgccag atcgttctcg ctaccgcaac caccggtatg aaccagattg gtgcgcacta
      241 tgaaggccac aagatcattg agattggtgc cgttgaagtg gtgaaccgtc gcctgacggg
      301 caataacttc catgtttatc tcaaacccga tcggctggtg gatccggaag cctttggcgt
      361 acatggtatt gccgatgaat ttttgctcga taagcccacg tttgccgaag tagccgatga
      421 gttcatggac tatattcgcg gcgcggagtt ggtgatccat aacgcagcgt tcgatatcgg
      481 ctttatggac tacgagtttt cgttgcttaa gcgcgatatt ccgaagacca atactttctg
      541 taaggtcacc gatagccttg cggtggcgag gaaaatgttt cccggtaagc gcaacagcct
      601 cgatgcgtta tgtgctcgct acgaaataga taacagtaaa cgaacgctgc acggggcatt
      661 actcgatgcc cagatccttg cggaagttta tctggcgatg accggtggtc aaacgtcgat
      721 ggcttttgcg atggaaggag agacaca
//
