LOCUS       sequence-125519-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH
  TITLE     Live cell imaging of Gs and the beta2-adrenergic receptor 
            demonstrates that both alphas and beta1gamma7 internalize upon 
            stimulation and exhibit similar trafficking patterns that differ 
            from that of the beta2-adrenergic receptor.
  JOURNAL   J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5.
  PUBMED    15297467
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-15"; volume: "279(42)"; pages: "44101-12. 
            Epub 2004 Aug 5"
COMMENT     Sequence Label: Beta-2-adrenergic receptor-mRFP
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..710
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             33..710
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(163..181)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     431..450
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     625..648
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atgactcact gctggatcca ccggtcgcca ccatggcctc ctccgaggac gtcatcaagg
       61 agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg
      121 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      181 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttccag tacggctcca
      241 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      301 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      361 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      421 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgga
      481 tgtaccccga ggacggcgcc ctgaagggcg agatcaagat gaggctgaag ctgaaggacg
      541 gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc gtgcagctgc
      601 ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag gactacacca
      661 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctaa agcggccgcg
      721 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
      781 cccacacctc cccctgaacc tgaaacataa aatgaatgca
//
