LOCUS       sequence-125523-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 NFKB1
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..46)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(189..208)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(190..208)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        240..344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             529..907
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(563..582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctcctcgcc cttgcttgtg ccgtggtgat ggtggtgatg gtggtgacca gaggtgccca
       61 tggtttcact tacgtgttta atgtctttac agactttata cattactgtc atggttaaat
      121 aaaataaaat aaaataaaat aaaataaaat aaaataaaat aaaataaaat aaaataaact
      181 taagatgtcc ctatagtgag tcgtattaga cgtcaggtgg cacttttcgg ggaaatgtgc
      241 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      301 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      361 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      421 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      481 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      541 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      601 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      661 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      721 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
      781 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
      841 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
      901 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
      961 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1021 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1081 cactgg
//
