LOCUS       sequence-125524-        1159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Standard for cell-free expression benchmarking..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y,
            Alexandrov K
  TITLE     Performance benchmarking of four cell-free protein expression 
            systems.
  JOURNAL   Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814.
  PUBMED    26301602
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814."
COMMENT     Sequence Label: pCellFree_G03 NFKB1
FEATURES             Location/Qualifiers
     source          1..1159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttccttcctg cagggtacca cgcgtaccca acacacggac acatgcacga acacgcatgc
       61 agagagatca tacatcatct atagagaagt acacgtaaaa gtaagactcg tcgtctgcca
      121 gctacaaccg agaaggcgac gtgaaaggaa caagaaagga ggaggagggc ggccgcttac
      181 accactttgt acaagaaagt tgggtaaatt ttgccttcta gaggtccttc ctgcccataa
      241 tcatggggca ttttgttgag agttagcagt gaggcaccac tggtcagaga ctcggtaaag
      301 ctgagtttgc ggaaggatgt ctccacgccg ctgtcgcaga cactgtcact gtctcggagc
      361 tcgtctattt gctgccttgt ggaggcaggc gagagaggca gcgagtgggc ctgagaggtg
      421 gtcttcactg ggctggaggc tgcctggatc acttcaattg cttcggtgta gcccatttgt
      481 ctcagggcct ccaccagctc tctgactgta cccccagaga cctcatagtt gtccataagt
      541 gttttggaag gagcaggact cagccggaag gcattattaa gtatccccag acctaatttc
      601 tgcgccagag tagcccagtt tttgtctgga tcaggaattt ctagtaactt atacagctgc
      661 agcttcacat cttcagccag ctgtttcatg tctccttgtg ctagtaaatc atcagatgta
      721 aactctggct catatggttt cccatttaat atgtcaaata cctgccagct ggtggccata
      781 tctagaggcg tggttccagg cacaactcct tcatcctctc ctgcattttc ccaagagtca
      841 tccaggtcat agagaggctc aaagttctcc accaggggat ctgctcctgc tgctttgaga
      901 agagctgcca gcctggtgga ccctctccca gctgctatat gcaggggtgt ggttccatcg
      961 taggtagtac tgtccacatg ggcatcaccc tccaggagca ggcagcctgc caatgagatg
     1021 ttgtcgtgct ccacagccag gtgcagtgct gtgcgcccgg acttctgctc ctgagcattg
     1081 acgtcagccc cagcggccac cagcagcagc aaacatggca ggctattgct catcatggct
     1141 agatgaatgg cattcagac
//
