LOCUS       sequence-125567-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR with FRT sites + pT7::SgrS::T1 terminator + ColE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Shin JE, Lin C, Lim HN
  TITLE     Horizontal transfer of DNA methylation patterns into bacterial 
            chromosomes.
  JOURNAL   Nucleic Acids Res. 2016 Apr 15. pii: gkw230.
  PUBMED    27084942
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 15. pii: gkw230."
COMMENT     Sequence Label: pHL 2146
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      244..330
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(575..594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcagcactat tttaccgcga cagcgaagtt gtgctggttg cgttggttaa gcgtcccaca
       61 acgattaacc atgcttgaag gactgatgca gtgggatgac cgcaattctg aaagttgact
      121 tgcctgcatc atgtgtgact gagtattggt gtaaaatcac ccgccagcag attatacctg
      181 ctggtttttt ttattctcgc cgcgctaaaa agcttgggcc catggtacgc gtgctagagg
      241 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      301 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctaggg cgttcggctg
      361 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      421 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      481 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      541 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      601 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      661 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      721 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      781 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      841 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      901 ttgaag
//
