LOCUS       sequence-125570-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized Lb3Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY005 (pcDNA3.1-hLb3Cpf1)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catggattac ggcaacggcc
      121 agtttgagcg gagagccccc ctgaccaaga caatcaccct gcgcctgaag cctatcggcg
      181 agacacggga gacaatccgc gagcagaagc tgctggagca ggacgccgcc ttcagaaagc
      241 tggtggagac agtgacccct atcgtggacg attgtatcag gaagatcgcc gataacgccc
      301 tgtgccactt tggcaccgag tatgacttca gctgtctggg caacgccatc tctaagaatg
      361 acagcaaggc catcaagaag gagacagaga aggtggagaa gctgctggcc aaggtgctga
      421 ccgagaatct gccagatggc ctgcgcaagg tgaacgacat caattccgcc gcctttatcc
      481 aggatacact gacctctttc gtgcaggacg atgccgacaa gcgggtgctg atccaggagc
      541 tgaagggcaa gaccgtgctg atgcagcggt tcctgaccac acggatcaca gccctgaccg
      601 tgtggctgcc cgacagagtg ttcgagaact ttaatatctt catcgagaac gccgagaaga
      661 tgagaatcct gctggactcc cctctgaatg agaagatcat gaagtttgac ccagatgccg
      721 agcagtacgc ctctctggag ttctatggcc agtgcctgtc tcagaaggac atcgatagct
      781 acaacctgat catctccggc atctatgccg acgatgaggt gaagaaccct ggcatcaatg
      841 agatcgtgaa ggagtacaat cagcagatcc ggggcgacaa ggatgagtcc ccactgccca
      901 agct
//
