LOCUS       sequence-125571-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized Lb3Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY005 (pcDNA3.1-hLb3Cpf1)
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..130)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(137..184)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg
       61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat
      121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      181 ttttgttgat cagctgggcc acgaaatcgg tccactctcc cctctccttg gagccgatcc
      241 tatagtagtc acacttccta ttcaggttca tggcctggat aaaggagata tcgtacttct
      301 tgccggtcac agggctatag tacacggccc tctgtcctgc cactccgctc actgtgccgc
      361 gcagataatt cagcaccaga gagtagatgg catccaccac cttgccaggc acgttctccc
      421 gctcattcag cttgttcagc tcggtgtctg tctcgatgcc attctcctcc agcacgcggg
      481 acagctcctc cacgggctcc tcgatgaact ccaccttctt tgtctcccgg tcatagtttg
      541 tgattgggtg attgcaacag cagttccaca cgcgattgtc ggtgttgtgt gtaggcaggg
      601 aatcgccgct gtacttcatg atcaggcagc cctcagagga caccttcagc tgatcgaact
      661 tgctcagaaa gttcagcttg gccttcttgg tcctgatatt gtggtcgttg atggcaaaga
      721 tgaagccggt gtcggggtcc aggctccgtg tcatagaatt tggcaccata aagatcacgc
      781 cgtcctgcag gatcttatta gaatcgttct ggcacagctg cagggggttt gtgaaggaac
      841 atggctcgcc gtccttgatg cccctaaagt gcagatcgct cagcttggcc agcagctttg
      901 tc
//
