LOCUS       sequence-125575-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR with FRT sites + pT7::LacO::SgrS::T1 terminator + ColE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Shin JE, Lin C, Lim HN
  TITLE     Horizontal transfer of DNA methylation patterns into bacterial 
            chromosomes.
  JOURNAL   Nucleic Acids Res. 2016 Apr 15. pii: gkw230.
  PUBMED    27084942
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 15. pii: gkw230."
COMMENT     Sequence Label: pHL 2147
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      280..366
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(611..630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggatgaagc aaggggtgcc ccatgcgtca gttttatcag cactatttta ccgcgacagc
       61 gaagttgtgc tggttgcgtt ggttaagcgt cccacaacga ttaaccatgc ttgaaggact
      121 gatgcagtgg gatgaccgca attctgaaag ttgacttgcc tgcatcatgt gtgactgagt
      181 attggtgtaa aatcacccgc cagcagatta tacctgctgg ttttttttat tctcgccgcg
      241 ctaaaaagct tgggcccatg gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag
      301 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
      361 acaaatccgc cgccctagac ctagggcgtt cggctgcggc gagcggtatc agctcactca
      421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
//
