LOCUS       sequence-125576-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized BpCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY006 (pcDNA3.1-hBpCpf1)
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgctgctg tatgagaact
      121 acaccaagcg gaatcagatc acaaagagcc tgaggctgga gctgcgccct cagggcaaga
      181 ccctgagaaa catcaaggag ctgaatctgc tggagcagga caaggccatc tacgccctgc
      241 tggagcggct gaagccagtg atcgatgagg gcatcaagga catcgccaga gataccctga
      301 agaactgcga gctgtctttt gagaagctgt acgagcactt cctgagcggc gacaagaagg
      361 cctatgccaa ggagtccgag cggctgaaga aggagatcgt gaaaaccctg atcaagaacc
      421 tgcccgaggg catcggcaag atctctgaga tcaacagcgc caagtatctg aatggcgtgc
      481 tgtacgactt catcgataag acacacaagg actctgagga gaagcagaat atcctgagcg
      541 atatcctgga gacaaagggc tacctggccc tgttctccaa gtttctgaca tctcggatca
      601 ccacactgga gcagtccatg cctaagagag tgatcgagaa cttcgagatc tatgccgcca
      661 atatcccaaa gatgcaggac gccctggaga ggggagccgt gtcttttgcc atcgagtacg
      721 agagcatctg ttccgtggat tactataacc agatcctgtc ccaggaggac atcgattctt
      781 ataatcgcct gatcagcggc atcatggacg aggatggcgc caaggagaag ggcatcaacc
      841 agaccatctc cgagaagaat atcaagatca agtctgagca cctgga
//
