LOCUS       sequence-125578-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PeCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY007 (pcDNA3.1-hPeCpf1)
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgtccaac ttctttaaga
      121 atttcaccaa cctgtatgag ctgtccaaga cactgaggtt tgagctgaag cccgtgggcg
      181 acaccctgac aaacatgaag gaccacctgg agtacgatga gaagctgcag accttcctga
      241 aggatcagaa tatcgacgat gcctatcagg ccctgaagcc tcagttcgac gagatccacg
      301 aggagtttat cacagattct ctggagagca agaaggccaa ggagatcgac ttctccgagt
      361 acctggatct gtttcaggag aagaaggagc tgaacgactc tgagaagaag ctgcgcaaca
      421 agatcggcga gacattcaac aaggccggcg agaagtggaa gaaggagaag taccctcagt
      481 atgagtggaa gaagggctcc aagatcgcca atggcgccga catcctgtct tgccaggata
      541 tgctgcagtt tatcaagtat aagaacccag aggatgagaa gatcaagaat tacatcgacg
      601 atacactgaa gggcttcttt acctatttcg gcggctttaa tcagaacagg gccaactact
      661 atgagacaaa gaaggaggcc tccaccgcag tggcaacaag gatcgtgcac gagaacctgc
      721 caaagttctg tgacaatgtg atccagttta agcacatcat caagcggaag aaggatggca
      781 ccgtggagaa aaccgagaga aagaccgagt acctgaacgc ctaccagtat ctgaagaaca
      841 ataacaagat cacacagatc aaggacgccg agacagagaa gatgatcgag tctacaccca
      901 tcgccgagaa gatcttcgac gtg
//
