LOCUS       sequence-125581-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized PeCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY007 (pcDNA3.1-hPeCpf1)
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..121)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(128..175)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cgagcggccg ccactgtgct ggatatctgc agaattctta ggcatagtcg gggacatcat
       61 atgggtatgc ataatcaggc acgtcgtagg gataagcgta atctggaaca tcgtatgggt
      121 aggatccctt tttctttttt gcctggccgg cctttttcgt ggccgccggc cttttcttct
      181 tgcgcttggc catggccttc ctgctagaaa agatgttcag ctgcttcttc cactcggtct
      241 tgttattcag gtgcagatcc cactcctcgt cagacacgaa cagggacaga tccttggagt
      301 cgctattggc caggatgtgg gcgttcatga tgatgccctt ccgggcgatg ttgaaggcgc
      361 cattggcatc gccggagctg ggcatgctga tcttctcgcc ctttgtctcc ttatcccagt
      421 actcgcgaga gtcaaagtgc ttgccattct cgtccctcac tggggacagg atgaaatcgt
      481 tgtctctctc gttattgccg gtattccgga tctgctggat caggttgatg gcgaatctca
      541 gggactcgcc tgtgccgtgc tcgttattcc gggtcagctc cacgccctcc ttcagctgct
      601 tcagcaggct gatgttcttg tcaaaattca cgaacagatc gtccaggatc ttcacgatat
      661 cgatcttgtc gatcttccac tcatacttat ccttctccct ctctccgcgg aacctgtcca
      721 gggacttgcc attctcgccg gagtacagcc gccacttctt gggcttgatc tcgccggttt
      781 tctcatccac aaactcgccc ttgtcgtact cgaaatagta atccttgccg tcaaagccga
      841 tatcggtgaa tgtctcgatg atctggtcct tcacgctctt gttcttgatc ttgccgtcct
      901 tcttgtatgt tgtctcctcg
//
