LOCUS       sequence-125583-         348 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient expression in mammalian cells of an ""INT"" 
            construct_bearing the ""Spinach2"" aptamer, targeting the GLuc 
            reporter. U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pU6_(GLuc)_INT[Spinach2]
FEATURES             Location/Qualifiers
     source          1..348
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..159
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
       61 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
      121 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
      181 cttggcttta tatatcttgt ggaaaggacg aaacaccgat ctagatacga ctcactatgt
      241 ttaagagcta tgctgcgaat acgaggatgt aactgaatga aatggtgaag gacgggtcca
      301 gtaggctgct tcggcagcct acttgttgag tagagtgtga gctccgta
//
