LOCUS       sequence-125588-        9387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Display Cypridina Luciferase-2A-Venus YFP ""normalizer,"" 
            lentiviral format..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
  TITLE     Multiplexable, locus-specific targeting of long RNAs with 
            CRISPR-Display.
  JOURNAL   Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 
            2015 Jun 1.
  PUBMED    26030444
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3433"; journalName: "Nat Methods"; date: "2015-07"; 
            volume: "12"; issue: "7"; pages: "664-70"
COMMENT     Sequence Label: pLenti_9xD3B_Cluc_Norm
FEATURES             Location/Qualifiers
     source          1..9387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1034..1052
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1090..1112)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1212..1231
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1425..1447
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1439..1456
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1440..1456
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1485..1711
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1712..1892
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1939..2064
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2557..2790
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2975..3019
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3286..3403
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3822..3860
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3857..3881
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3907..5565
                     /codon_start=1
                     /product="secreted Cypridina luciferase"
                     /label=CLuc
                     /note="contains 30 codon substitutions for efficient 
                     translation in mammalian cells"
                     /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECIDS
                     SCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTYVL
                     GQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADPIIA
                     NPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLCGDLK
                     MMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPINFYYY
                     TISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQFQGPCK
                     EILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVGGEAVSVP
                     YSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGICGNYNQDFS
                     DDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHKPDRVERCMY
                     EYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK"
     CDS             5590..5643
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5650..5652,5653..5655,5656..6366)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5695..5716)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5956..5975)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6303..6324
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6376..6432
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6439..7038
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6439..6458)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6935..6955
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7065..7653
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7118..7138)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7536..7547)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7759..7992
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8064..8185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8101..8120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8155..8174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8234..8250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8234..8250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8247..8269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8258..8274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8282..8288,8289..8306,8307..8312))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8327..8348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8458..8475)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8629..9217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8709..8728)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     1081 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     1141 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     1201 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     1261 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     1321 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1381 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1441 taaaacgacg gccagtgaat tcgagctcgg taccttctcg aaaatgtagt cttatgcaat
     1501 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     1561 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     1621 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     1681 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     1741 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     1801 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     1861 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     1921 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     1981 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     2041 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     2101 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     2161 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     2221 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     2281 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     2341 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     2401 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2461 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2521 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2581 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2641 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2701 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2761 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2821 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     2881 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     2941 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3001 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3061 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3121 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3181 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3241 cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta aaagaaaagg
     3301 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     3361 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatagggc ccgggcatct
     3421 ggctgggtac acaccccccc atatttctgt gctctgccac ccgtgagctg ccactttccc
     3481 tcgagctttg cggtaatcta gatcgcccgt cccctaggca aagctctaat ctagatcgcc
     3541 cgtcccctag gaaaggaagc agccacatct agatcgcccg tcccctagga taacgttaga
     3601 tctagatcgc ccgtccccta gggtgaagta gtctttgcgg taatctagat cgcccgtccc
     3661 ctaggcaaag ctctaatcta gatcgcccgt cccctaggaa aggaagcagc cacatctaga
     3721 tcgcccgtcc cctaggataa cgttagatct agatcgcccg tcccctaggg tgaagtagta
     3781 tctagatcgc ccgtccccta ggccggatcc acgtatgtcg aggtaggcgt gtacggtggg
     3841 aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggagg taccgccacc
     3901 gccaccatga agaccttaat tcttgccgtt gcattagtct actgcgccac tgttcattgc
     3961 caggactgtc cttacgaacc tgatccacca aacacagttc caacttcctg tgaagctaaa
     4021 gaaggagaat gtattgatag cagctgtggc acctgcacga gagacatact atcagatgga
     4081 ctgtgtgaaa ataaaccagg aaaaacatgt tgccgaatgt gtcagtatgt aattgaatgc
     4141 agagtagagg ccgcaggatg gtttagaaca ttctatggaa agagattcca gttccaggaa
     4201 cctggtacat acgtgttggg tcaaggaacc aagggcggcg actggaaggt gtccatcacc
     4261 ctggagaacc tggatggaac caagggggct gtgctgacca agacaagact ggaagtggct
     4321 ggagacatca ttgacatcgc tcaagctact gagaatccca tcactgtaaa cggtggagct
     4381 gaccctatca tcgccaaccc gtacaccatc ggcgaggtca ccatcgctgt tgttgagatg
     4441 ccaggcttca acatcaccgt cattgagttc ttcaaactga tcgtgatcga catcctcgga
     4501 ggaagatctg taagaatcgc cccagacaca gcaaacaaag gaatgatctc tggcctctgt
     4561 ggagatctta aaatgatgga agatacagac ttcacttcag atccagaaca actcgctatt
     4621 cagcctaaga tcaaccagga gtttgacggt tgtccactct atggaaatcc tgatgacgtt
     4681 gcatactgca aaggtcttct ggagccgtac aaggacagct gccgcaaccc catcaacttc
     4741 tactactaca ccatctcctg cgccttcgcc cgctgtatgg gtggagacga gcgagcctca
     4801 cacgtgctgc ttgactacag ggagacgtgc gctgctcccg aaactagagg aacctgcgtt
     4861 ttgtctggac atactttcta cgatacattt gacaaagcaa gataccaatt ccagggtccc
     4921 tgcaaggaga ttcttatggc cgccgactgt ttctggaaca cttgggatgt gaaggtttca
     4981 cacaggaatg ttgactctta cactgaagta gagaaagtac gaatcaggaa acaatcgact
     5041 gtagtagaac tcattgttga tggaaaacag attctggttg gaggagaagc cgtgtccgtc
     5101 ccgtacagct ctcagaacac ttccatctac tggcaagatg gtgacatact gactacagcc
     5161 atcctacctg aagctctggt ggtcaagttc aacttcaagc aactgctcgt cgtacatatt
     5221 agagatccat tcgatggtaa gacttgcggt atttgcggta actacaacca ggatttcagt
     5281 gatgattctt ttgatgctga aggagcctgt gatctgaccc ccaacccacc gggatgcacc
     5341 gaagaacaga aacctgaagc tgaacgactc tgcaatagtc tcttcgccgg tcaaagtgat
     5401 cttgatcaga aatgtaacgt gtgccacaag cctgaccgtg tcgaacgatg catgtacgag
     5461 tattgcctga ggggacaaca gggtttctgt gaccacgcat gggagttcaa gaaagaatgc
     5521 tacataaagc atggagacac cctagaagta ccagatgaat gcaaaggcag caccagcggc
     5581 ggcagtggag agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     5641 ccaggcagta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     5701 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     5761 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     5821 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
     5881 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5941 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     6001 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     6061 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     6121 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     6181 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6241 gacaaccact acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     6301 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     6361 tacaagggca gcggtgctac gaacttcagt ctcctgaaac aggctgggga tgtggaggag
     6421 aaccccggcc caggcagcat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6481 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6541 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6601 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6661 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6721 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6781 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6841 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6901 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6961 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7021 cgcaagcccg gtgcctagta acgtaccggt tagtaatgat cgacaatcaa cctctggatt
     7081 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7141 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7201 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7261 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7321 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7381 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7441 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     7501 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7561 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7621 cgagtcggat ctccctttgg gccgcctccc cgcctggcga tggtaccgct aagcacaatt
     7681 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7741 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7801 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7861 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7921 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7981 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8041 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8101 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8161 tgtccaaact catcaatgta tcttatcatg tctgggttta aacctgcagg catgcaagct
     8221 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8281 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8341 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8401 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg ccgcttcctc
     8461 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8521 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8581 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8641 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8701 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8761 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8821 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8881 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8941 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9001 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9061 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9121 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9181 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9241 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9301 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9361 tatatatgag taaacttggt ctgacag
//
