LOCUS       sequence-125589-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized LPbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY008 (pcDNA3.1-hPbCpf1)
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..130)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(137..184)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg
       61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat
      121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      181 ttttgtggtc caggttcagg gctgtggtgc ctgcgtactt gccccaatcc cgggctgcct
      241 cgtcccactc tgcattgctg ataaacaggt ttggcagctt cttgaactcg gggtccttct
      301 tggcgtgctc gcggatcttc ttcaggatca tcacgccctt cctggcgata ttataggctc
      361 cgtttgcgtc tgcattctcc acggccagcc agcacaggtt ggaggggatg taagggttgg
      421 gtgtggtaaa gaagggcttc actgggctgg cgatgaagtc cacgccctcg tccactgcga
      481 tcacttctcc tgccttgatc ttgatctgca ggctgaaaga attgcgcagc tccagcacca
      541 ggttaaacag gtagatgaaa gagtgccaaa agtttctcag ccggccatcc agctccttct
      601 ctccctgcag gtcgcctgcc ttctccttct tgatgatctc ctgcttgata tctgtggcct
      661 ttgggttcac aaaattccag accttcagca gcttctcgaa ctcctcattc actttcaccc
      721 tatcatactt ccagtagccg tcctcgccct tctgtctccg gattcttggg gcgctggcaa
      781 agatggccca ctccttgctc acggtagagt tcttatactt ctcgtcggcc aggttgtaag
      841 g
//
