LOCUS       sequence-125590-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized LPbCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY008 (pcDNA3.1-hPbCpf1)
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catggagaac atcttcgacc
      121 agtttatcgg caagtacagc ctgtccaaga ccctgagatt cgagctgaag cccgtgggca
      181 agacagagga cttcctgaag atcaacaagg tgtttgagaa ggatcagacc atcgacgata
      241 gctacaatca ggccaagttc tattttgatt ccctgcacca gaagtttatc gacgccgccc
      301 tggcctccga taagacatcc gagctgtctt tccagaactt tgccgacgtg ctggagaagc
      361 agaataagat catcctggat aagaagagag agatgggcgc cctgaggaag cgcgacaaga
      421 acgccgtggg catcgatagg ctgcagaagg agatcaatga cgccgaggat atcatccaga
      481 aggagaagga gaagatctac aaggacgtgc gcaccctgtt cgataacgag gccgagtctt
      541 ggaaaaccta ctatcaggag cgggaggtgg acggcaagaa gatcaccttc agcaaggccg
      601 acctgaagca gaagggcgcc gattttctga cagccgccgg catcctgaag gtgctgaagt
      661 atgagttccc cgaggagaag gagaaggagt ttcaggccaa gaaccagccc tccctgttcg
      721 tggaggagaa ggagaatcct ggccagaaga ggtacatctt cgactctttt gataagttcg
      781 ccggctatct gaccaagttt cagcagacaa agaagaatct gtacgcagca gacggcacca
      841 gcacagcagt ggccacccgc atcgccgata actttatcat cttccaccag aataccaagg
      901 tgttccg
//
