LOCUS       sequence-125594-        8856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral cDNA/shRNA gateway destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Radulovich N, Leung L, Tsao MS
  TITLE     Modified gateway system for double shRNA expression and Cre/lox 
            based gene expression.
  JOURNAL   BMC Biotechnol. 2011 Mar 22;11:24.
  PUBMED    21418658
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2011-03-22"; volume: "11"; pages: "24"
COMMENT     Sequence Label: pLKO.DEST.EGFP
FEATURES             Location/Qualifiers
     source          1..8856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(114..238)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    318..435
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        524..1023
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(559..582)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     956..975
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(1048..1067)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             join(1180..1182,1183..1185,1186..1899)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1180..1182,1183..1185,1186..1896)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1225..1246)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1486..1505)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1833..1854
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1989..2222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2294..2415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2331..2350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2385..2404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2455..2590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2731..3186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2818..2837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3028..3049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3212..3316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3317..3385,3386..4177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3535..3554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4767..4786
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5020..5037
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5190..5206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5190..5206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5225..5245
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5227..5245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5273..5499
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5500..5680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5727..5852
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6345..6578
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6763..6807
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7105..7353
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7105..7125
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7276..7295
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    7390..7514
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7539..7544,7545..7562,7563..7569)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7623..8282
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7689..7708)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             join(8624..8856,1..73)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8808..8827
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
       61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
      121 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
      181 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
      241 atgggcggcc gctctagagg gcccaagctt acgcgtgaat tctcgacctc gagacaaatg
      301 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
      361 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      421 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc
      481 gagggggttg gggttgcgcc ttttccaagg ctagaattct accgggtagg ggaggcgctt
      541 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
      601 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
      661 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
      721 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
      781 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
      841 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
      901 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
      961 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     1021 tcgacctgca gcccaagcta gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     1081 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     1141 gccacgcgcc acaccgtcga tccggaccga accacaacca tggtgagcaa gggcgaggag
     1201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1321 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1381 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1621 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1681 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1741 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1861 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcat atgcatcggt
     1921 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     1981 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     2041 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2101 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     2161 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     2221 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     2281 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2341 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2401 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     2461 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     2521 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     2581 tttggaggcc tagggacgta cccaattcgc ctggcgttac ccaacttaat cgccttgcag
     2641 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2701 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     2761 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2821 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2881 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2941 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3001 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3061 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3121 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3181 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3241 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3301 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3361 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3421 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3481 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3541 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3601 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3661 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3721 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3781 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3841 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3901 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3961 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4021 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4081 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4141 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4201 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4261 ttttgataat ctcatgagga agcggaagag cgctgcgcgt aatctgctgc ttgcaaacaa
     4321 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4381 cgaaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4441 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4501 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4561 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4621 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4681 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4741 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4801 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4861 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4921 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4981 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5041 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5101 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5161 agttagctca ctcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     5221 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
     5281 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
     5341 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
     5401 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
     5461 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
     5521 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5581 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5641 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     5701 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     5761 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     5821 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     5881 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     5941 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     6001 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     6061 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     6121 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     6181 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     6241 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6301 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6361 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6421 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6481 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     6541 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6601 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     6661 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     6721 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     6781 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     6841 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     6901 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     6961 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     7021 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag
     7081 cctcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc atatttgcat
     7141 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
     7201 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     7261 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     7321 gctttatata tcttgtggaa aggacgaaac accggtccgc aggtatgcac gcgatcctcg
     7381 agcccatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     7441 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     7501 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     7561 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     7621 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     7681 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     7741 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     7801 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     7861 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     7921 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     7981 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     8041 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     8101 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     8161 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc
     8221 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     8281 aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     8341 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc
     8401 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     8461 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     8521 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     8581 gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta
     8641 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     8701 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     8761 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     8821 cgatatggcc agtgtgccgg tctccgttat cgggga
//
