LOCUS       sequence-125595-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  detection/assessment of OCT4 and SOX2 transcription factor activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  TITLE     luciferase reporter plasmid containing 6 tandem copies of the 
            composite Oct4/Sox2 binding site of the FGF4 enhancer. Used for 
            detection of active Oct4/Sox2 complexes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 6xO/S luc
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(512..528)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(512..528)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    591..712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(602..621)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     656..675
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc attttaccaa cagtaccgga
       61 atgccaagct tacttagatc ttgcgtgagt tcgagctgcg ggccgtgagt tcggtgagtg
      121 ctgcgcacag acctaagtag cgagagcaaa tagggacgca acggtggcgg gatagtcccc
      181 gcgcgcccag ccctccggag cagtggtata tatagccggc gccccctcct actcgcctgc
      241 cccgcgcccg ggggatcctt taagtatccc attagcatcc aaacaaagag ttttcagatc
      301 ctttaagtat cccattagca tccaaacaaa gagttttcag atcctttaag tatcccatta
      361 gcatccaaac aaagagtttt cagatccttt aagtatccca ttagcatcca aacaaagagt
      421 tttcagatcc tttaagtatc ccattagcat ccaaacaaag agttttcaga tcctttaagt
      481 atcccattag catccaaaca aagagttttc agatccacta gttctagagc ggccgccacc
      541 gcggtggagc tcggtacctc ccgggttatg ttagctcagt tacagtacca taagatacat
      601 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
      661 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
      721 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
      781 gtacaacctc cacgtacctt aatattactt acttatcatg gtagcttggg ctggcgtaat
      841 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      901 caaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
      961 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccga
//
