LOCUS       sequence-125596-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express humanized Cas9 under the control of trpC promoter and 
            terminator from A. nidulans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p415-PtrpC-Cas9-TtrpC-CYC1t
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(119..686)
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene"
     CDS             complement(858..878)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg
       61 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tactctagaa
      121 agaaggatta cctctaaaca agtgtacctg tgcattctgg gtaaacgact cataggagag
      181 ttgtaaaaaa gtttcggccg gcgtattggg tgttacggag cattcactag gcaaccatgg
      241 ttactattgt atacccatct tagtaggaat gattttcgag gtttatacct acgatgaatg
      301 tgtgtcctgt aggcttgaga gttcaaggaa gaaacagtgc aattatcttt gcgaacccag
      361 gggctggtga cggaattttc atagtcaagc tatcagagta aagaagagga gcatgtcaaa
      421 gtacaattag agacaaatat atagtcgcgt ggagccaaga gcggattcct cagtctcgta
      481 ggtctcttga cgaccgttga tctgcttgat ctcgtctccc gaaaatgaaa atagactctg
      541 ctaagctatt cttctgcttc gccggagcct gaagggcgta ctagggttgc gaggtccaat
      601 gcattaatgc attgcagatg agctgtatct ggaagaggta aacccgaaac gcgttttatt
      661 cttgttgaca tggagctatt aaatcactag aaggcactct ttgctgcttg gacaaatgaa
      721 cgtatcttat cgagatcctg aacaccattt gtctcaactc cggagctgac atcgacacca
      781 acgatcttat atccagattc gtcaagctgt ttgatgattt cagtaacgtt aagtacgcgt
      841 ctcgaggtcc aaaatcacac cttcctcttc ttcttgggg
//
