LOCUS       sequence-125598-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  basal plasmid containing minimal fgf4 promoter used for testing 
            enhancer activity of DNAs inserted immediately upstream of the 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Murtha M, Tokcaer-Keskin Z, Tang Z, Strino F, Chen X, Wang Y, Xi X, 
            Basilico C, Brown S, Bonneau R, Kluger Y, Dailey L
  TITLE     FIREWACh: high-throughput functional detection of transcriptional 
            regulatory modules in mammalian cells.
  JOURNAL   Nat Methods. 2014 May;11(5):559-65. doi: 10.1038/nmeth.2885. Epub 
            2014 Mar 23.
  PUBMED    24658142
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2885"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "559-65"
COMMENT     Sequence Label: fgfprom luc
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      290..299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(362..380)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 cagaacattt ctctatcgat aggtaccgag ctcttacgcg tgctagcccg ggctcgagat
       61 ctcgagctct tacgcgtgct agcccagcac ccgggcgcgg ggcaggcgag taggaggggg
      121 cgccggctat atataccact gctccggagg gctgggcgcg cggggactat cccgccaccg
      181 ttgcgtccct atttgctctc gctacttagg tctgtgcgca gcactcaccg aactcacggc
      241 ccgcagctcg aactcacgca aagcttggca ttccggtact gttggtaaag ccaccatgga
      301 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
      361 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
      421 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
      481 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
      541 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
      601 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
      661 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
      721 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
      781 gtcgatatac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
      841 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
      901 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
      961 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     1021 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gat
//
