LOCUS       sequence-125599-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  basal plasmid containing minimal fgf4 promoter used for testing 
            enhancer activity of DNAs inserted immediately upstream of the 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Murtha M, Tokcaer-Keskin Z, Tang Z, Strino F, Chen X, Wang Y, Xi X, 
            Basilico C, Brown S, Bonneau R, Kluger Y, Dailey L
  TITLE     FIREWACh: high-throughput functional detection of transcriptional 
            regulatory modules in mammalian cells.
  JOURNAL   Nat Methods. 2014 May;11(5):559-65. doi: 10.1038/nmeth.2885. Epub 
            2014 Mar 23.
  PUBMED    24658142
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2885"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "559-65"
COMMENT     Sequence Label: fgfprom luc
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    323..414
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(355..374)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    428..476
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(744..765)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     956..975
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta
       61 ccggaatgcc aagctttgcg tgagttcgag ctgcgggccg tgagttcggt gagtgctgcg
      121 cacagaccta agtagcgaga gcaaataggg acgcaacggt ggcgggatag tccccgcgcg
      181 cccagccctc cggagcagtg gtatatatag ccggcgcccc ctcctactcg cctgccccgc
      241 gcccgggtgc tgggctagca cgcgtaagag ctcgagatct cgagcccggg ctagcacgcg
      301 taagagctcg gtacctatcg atagagaaat gttctggcac ctgcacttgc actggggaca
      361 gcctattttg ctagtttgtt ttgtttcgtt ttgttttgat ggagagcgta tgttagtact
      421 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
      481 ccgctccaag tacctcccgt accttaatat tacttactta tcatggtagc ttgggctggc
      541 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      601 aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
      661 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      721 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      781 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      841 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      901 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      961 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1021 ccaccacacc cgccgcgctt aatgcgccgc tacagggc
//
