LOCUS       sequence-125601-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-On system expressing GFP tagged with 2x nuclear localization 
            signals..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Gersbach
  TITLE     TMG reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: tetO-GFPnls-mPGK-rtTA-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(55..73,74..96,97..115,116..138,139..157,158..180,
                     181..199,200..222,223..241,242..264,265..283,284..306,
                     307..325)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        358..396
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     393..417
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             547..600
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(646..667)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagttta ccactcccta
       61 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      121 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      181 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      241 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      301 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
      361 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      421 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      481 cgcggccccg aattcgagct cggtacccgg ggatcctcta gtcagctgac gcgtttggtt
      541 atgcatgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
      601 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      661 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgcc
//
