LOCUS       sequence-125603-        3553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the Agrobacterium tumefaciens Nopaline synthase terminator 
            as a level 0 GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pTnos (GB0037)
FEATURES             Location/Qualifiers
     source          1..3553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     176..194
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(229..481)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(603..621)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(604..621)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(639..655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(639..655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(652..674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    663..679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(687..693,694..711,712..717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    732..753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(870..887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1041..1629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1121..1140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1800..2591,2592..2660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2423..2442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2661..2765)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2843..3298)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2980..3001)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3192..3211
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3424..3446
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3438..3455
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3439..3455
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3462..3481
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3462..3480
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3511..3520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3515..3524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgagcgcg agtcggtccc attattgaag catttatcag ggttattgtc tcatgagcgg
       61 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      121 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      181 gcgtatcacg aggccctttc gtcttcaaga attagcttgg caattcccga tctagtaaca
      241 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
      301 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
      361 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatcg
      421 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
      481 cgggatcgaa gatccattcc aagccgagac cactcatcgc ccaatcacta gtgaattcgc
      541 ggccgcctgc aggtcgacca tatgggagag ctcccaacgc gttggatgca tagcttgagt
      601 attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga
      661 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      721 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      781 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      841 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
      901 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      961 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1021 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1081 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1141 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1201 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1261 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1321 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1381 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1441 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1501 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1561 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1621 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1681 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1741 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1801 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     1861 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     1921 agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac
     1981 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2041 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2101 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2161 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2221 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2281 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2341 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2401 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2461 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2521 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2581 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2641 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2761 ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt
     2821 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
     2881 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     2941 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     3001 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgctggc
     3061 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     3121 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     3181 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     3241 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     3301 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     3361 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     3421 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     3481 gcgaattggg cccgacgtcg catgctcccg gccgccatgg cggccgcggg aattcgattg
     3541 ggcgatgagt ggt
//
