LOCUS       sequence-125606-         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-On system expressing MyoD-2A-GFP-2xNLS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Gersbach
  TITLE     TMG reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(55..73,74..96,97..115,116..138,139..157,158..180,
                     181..199,200..222,223..241,242..264,265..283,284..306,
                     307..325)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        358..396
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     393..417
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      535..544
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagttta ccactcccta
       61 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      121 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      181 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      241 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      301 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcgnnaccc gggtcgaggt
      361 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      421 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      481 cgcggccccg aattcgagct cggtacccgg ggatcctcta gtcagctgac gcgtgccacc
      541 atggagcttc tatcgccgcc actccgggac atagacttga caggccccga cggctctctc
      601 tgctcctttg agacagcaga cgacttctat gatgatccgt gtttcgactc accag
//
