LOCUS       sequence-125607-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized SsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY009 (pcDNA3.1-hSsCpf1)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgcagacc ctgtttgaga
      121 acttcacaaa tcagtaccca gtgtccaaga ccctgcgctt tgagctgatc ccccagggca
      181 agacaaagga cttcatcgag cagaagggcc tgctgaagaa ggatgaggac cgggccgaga
      241 agtataagaa ggtgaagaac atcatcgatg agtaccacaa ggacttcatc gagaagtctc
      301 tgaatggcct gaagctggac ggcctggaga agtacaagac cctgtatctg aagcaggaga
      361 aggacgataa ggataagaag gcctttgaca aggagaagga gaacctgcgc aagcagatcg
      421 ccaatgcctt ccggaacaat gagaagttta agacactgtt cgccaaggag ctgatcaaga
      481 acgatctgat gtctttcgcc tgcgaggagg acaagaagaa tgtgaaggag tttgaggcct
      541 tcaccacata cttcaccggc ttccaccaga accgcgccaa tatgtacgtg gccgatgaga
      601 agagaacagc catcgccagc aggctgatcc acgagaacct gccaaagttt atcgacaata
      661 tcaagatctt cgagaagatg aagaaggagg cccccgagct gctgtctcct ttcaaccaga
      721 ccctgaagga tatgaaggac gtgatcaagg gcaccacact ggaggagatc tttagcctgg
      781 attatttcaa caagaccctg acacagagcg gcatcgacat ctacaattcc gtgatcggcg
      841 gcagaacccc tgaggagggc aagacaaaga tca
//
