LOCUS       sequence-125609-        3890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides the S. Lycopersicum 2A11 terminator as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pT2A11 (GB0143)
FEATURES             Location/Qualifiers
     source          1..3890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(940..958)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(941..958)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(976..992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(976..992)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(989..1011)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1000..1016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1024..1030,1031..1048,1049..1054))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1069..1090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1207..1224)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1378..1966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1458..1477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2137..2928,2929..2997))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2760..2779
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2998..3102)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3180..3635)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3317..3338)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3529..3548
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3761..3783
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3775..3792
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3776..3792
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3799..3818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3799..3817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3848..3857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3852..3861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcggcttac aatatcaatg atctatcgat cgatctatct atctatttat ctgtctctgc
       61 gcgtatagtg ttgtctgtac ctttggtgtg aagaatatga ataaagggat acatatatct
      121 agatatattc taggtaatgt cctattgtat ttaaaatttg tagcaatgat tgtttgaata
      181 aaaacatacc atgagtgaaa taattattcc acattaattc acgtatttat ttcacttatg
      241 atacgtattt ttgttccttt cgcgtagatt tttgatcctt ttcccttttg aatattaaac
      301 attaaacaca aataatgttt attaaattaa gttaatattt ttatttagct atttatattt
      361 ttatttgaaa tcaaacttga taaatattta taaagataat taacaagtaa tgtgacacta
      421 acaccatgta atattatctt gtcgttattt atgataatat tttaaaatta taatttcagt
      481 taaaaaatta ttaaaaaaac atacttttaa aaagtgagtt agcctccgct acccacatac
      541 ttatgaattg gactagttgt tttttgaccc acaaaaagaa tgggctaatt aaacctgacc
      601 tatcaaattt cagaatctgc atagattagt ccgaacgaaa tgagtcagcc cgtattgaac
      661 aaaatatcaa caaggacgtt atgtaaagat gtttaagaag gaaaaaagat ttctaataca
      721 tatggacttt caatatccca actttgtctg gcgatctgaa ccctgcttag tttgttgatc
      781 attaacttgt cttgctatgt atttaagatt taaactttat atgtttaaac ttacagacgc
      841 tcgagaccac tcatcgccca atcactagtg aattcgcggc cgcctgcagg tcgaccatat
      901 gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc acctaaatag
      961 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1021 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1081 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1141 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1201 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1261 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1321 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1381 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1441 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1501 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1561 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1621 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1681 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1741 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1801 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1861 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1921 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1981 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2041 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2101 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2161 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2221 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2281 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2341 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2401 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2461 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2521 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2581 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2641 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2701 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2761 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2821 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2881 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2941 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3001 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3061 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3121 gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     3181 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     3241 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     3301 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     3361 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     3421 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     3481 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     3541 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     3601 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     3661 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     3721 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     3781 acgacggcca gtgaattgta atacgactca ctatagggcg aattgggccc gacgtcgcat
     3841 gctcccggcc gccatggcgg ccgcgggaat tcgattgggc gatgagtggt
//
