LOCUS       sequence-125610-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized SsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY009 (pcDNA3.1-hSsCpf1)
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..122)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(129..176)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcgagcggcc gccactgtgc tggatatctg cagaattctt aggcatagtc ggggacatca
       61 tatgggtatg cataatcagg cacgtcgtag ggataagcgt aatctggaac atcgtatggg
      121 taggatccct ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttgccc
      181 ttcagtgtct gcacgaactc cagccactcc ttgttggaga tggccagctt caccttcttc
      241 aggtcatcgg tcttgctgat ctgctccaga caccacaggc ccttcagggc gatgtgatag
      301 gcgccgttgg cgtcggcatt ctttggcatg tcatcgtcgg ccttccggga gtcaaagaag
      361 cggcccttag aatcggccac aggggacagg atgtagtcct gctcattatc gcccttctcg
      421 ccgttattgt gtctcagaga cagggtgatg gacaggttct tcatcagggc cttaaagaag
      481 tcggcggact cctggctggc gatctgctgc ttcagatcct tgccagactt atagtccacc
      541 ttgccatcga acagggactt cagctcggct gtgatgtcgt acttctcctg gctgcccctg
      601 ttattgttca gggccctatt ccaggcatat ctgtcctcgt ttgtggtgca cactgtccac
      661 ttggttctgc cgccatcggc cttctcggtg aaattcttga agtcaaaggc gaactcaaag
      721 taatcggcct tgctgttcag ccggatagag tcaaacttct caaagaaatc cttggcctga
      781 ttcaggttct catagcgggg cttcaggaag tcgataaagc cggtggcagg atctgtctta
      841 gaggtattcc aggcgggcac gtagaagata aagccgttct gcttgcccat ctt
//
