LOCUS       sequence-125611-         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-On system expressing MyoD-2A-GFP-2xNLS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Gersbach
  TITLE     TMG reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..124)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(131..151)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(194..215)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     543..562
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc aaccccaacc ccgtgcgaat
       61 tcgatatcgt ccacaagctt atcgatgcgg ccgcgctagc tcacaccttt ctctttttct
      121 tagggccacc caccttcctc ttcttcttgg gcttgtacag ctcgtccatg ccgagagtga
      181 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      241 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      301 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
      361 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
      421 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      481 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      541 gggtcttgta gttgccgtcg tccttgaaga agatggtgc
//
