LOCUS       sequence-125612-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express humanized Cas9 under the control of trpC promoter and 
            terminator from A. nidulans..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Matsu-Ura T, Baek M, Kwon J, Hong C
  TITLE     Efficient gene editing in Neurospora crassa with CRISPR technology.
  JOURNAL   Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 
            10.1186/s40694-015-0015-1. eCollection 2015.
  PUBMED    28955455
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2015-07-15"; volume: "2:4. doi"; pages: " 
            10.1186/s40694-015-0015-1. eCollection 2015"
COMMENT     Sequence Label: p415-PtrpC-Cas9-TtrpC-CYC1t
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..118)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttagtcagat tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa
       61 taatttttca ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt
      121 ttatcggatc ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc
      181 acgatatgat ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac
      241 atgtccctgc cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt
      301 tcaactgggt gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc
      361 ttcatccttt ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc
      421 tcgataacga tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc
      481 ttaacggtct gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc
      541 tcgtgaagac tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg
      601 agagagtcat catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga
      661 aaatccagga ttgtctttcc actctgcttg tctcggatcc cattgatcag ttttcttgac
      721 agccgccccc atcctgtata tcggcgcctc ttgagctgtt tcatgacttt gtcgtcgaag
      781 agatgagcgt aagttttcaa gcgttcttca atcatctccc tatcttcaaa caacgtaagg
      841 gtgaggacaa tgtcctcaag aatgtcctcg ttctcctcat tgtccaggaa gtccttgtct
      901 ttaatgattt tcaggagatc gtgatacgtt cccagggatg cgttgaa
//
