LOCUS       sequence-125613-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized Lb2Cpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY011 (pcDNA3.1-hLb2Cpf1)
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..130)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(137..184)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcttag gcatagtcgg
       61 ggacatcata tgggtatgca taatcaggca cgtcgtaggg ataagcgtaa tctggaacat
      121 cgtatgggta ggatcccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      181 ttttcagcag gtgtgtctgg gcatactcca gccactcggc gttggtcatg gccagattga
      241 tcttctcgcc ctcgctcttc tgcctgatct gctccagcac ccacaggccc tttctggcga
      301 tattgtaggc gccgttggca tcggcgtcct ttggcacaga gccgtcggac agctcgctgt
      361 tgaagtaggc ctctctctta ttcttcacag gggagatgat gtaatccctt gtgccgtcgg
      421 aggtgctgtt tctcatctgc agggtctgct gcagcagcct atacagtctc cggtagaact
      481 cggcctcctc gttgctgatg atcatgtcct tcacatttct gccatcctca taggggatct
      541 tgtactgctc aaacaggttc ttcatctcat cggtcaccac caccaccttc tcgtcgaaca
      601 tattgttctt atctggattc cgatacttga tgatgcgctc gccgttggtg cacacggtcc
      661 acttggaatt gatgccgcag gccctctggg tgaagctccg gtagtcaaag ccgaactcga
      721 acacgttctc cagggcgttg aacctgatga aatcaaagcc gtcaaagaat ctcttggact
      781 tctccacgtt ctcacacttc atataaaaca gattggcgaa gcctgtggtt ggatcgatct
      841 tagaggtcag gtaggcaggc acatagtaga tcacgccgga ctgcttgccc agctccttaa
      901 agctcttgaa cttagaggtc agctgcagtg cgttcagggc gcctcccagc tccttaggg
//
